In a recent publication in the Journal of Microbiological Methods, The Santa Maria Lab, led by first author Dr. Khomtchouk, describes a flow cytometry method to identify biofilms from planktonic bacteria. Using a combination of membrane permeable (SYTO 60) and impermeable (TOTO-1) dyes plus the addition of polystyrene counting beads we show the ability to identify biofilms in a way that simplifies biofilm diagnosis through a more rapid assessment.
